Effect of D614G Spike Variant on Immunoglobulin G, M, or A Spike Seroassay Performance

J Infect Dis. 2021 Mar 3;223(5):802-804. doi: 10.1093/infdis/jiaa743.

Abstract

Emergence of a new spike protein variant (D614G) with increased infectivity has prompted many to analyze its role in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. There is concern regarding whether an individual exposed to one variant of a virus will have cross-reactive memory to the second variant. Accordingly, we analyzed the serologic reactivity of both variants, and we found that antibodies from 88 donors from a high-incidence population reacted toward both the original spike and the D614 spike variant. These data suggest that patients who are exposed to either variant have cross-responsive humoral immunity. This represents an important finding both for SARS-CoV-2 disease biology and for therapeutics.

Keywords: COVID-19; D614G; SARS-CoV-2; coronavirus; pandemic; spike protein.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral / blood
  • COVID-19 / diagnosis*
  • COVID-19 / immunology
  • COVID-19 / virology
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Immunoglobulin A / blood*
  • Immunoglobulin G / blood*
  • Immunoglobulin M / blood*
  • Mutation
  • SARS-CoV-2 / classification
  • SARS-CoV-2 / immunology*
  • SARS-CoV-2 / pathogenicity
  • Spike Glycoprotein, Coronavirus / genetics
  • Spike Glycoprotein, Coronavirus / immunology*

Substances

  • Antibodies, Viral
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Spike Glycoprotein, Coronavirus
  • spike protein, SARS-CoV-2