Identification of the fatty acid coenzyme-A ligase FadD1 as an interacting partner of FptX in the Pseudomonas aeruginosa pyochelin pathway

FEBS Lett. 2021 Feb;595(3):370-378. doi: 10.1002/1873-3468.14012. Epub 2020 Dec 13.

Abstract

Pseudomonas aeruginosa is one of the most important nosocomial bacteria emerging as a highly multidrug-resistant pathogen. P. aeruginosa produces two siderophores including pyochelin (PCH) to fulfil its need for iron during infections. We know that both outer and inner membrane proteins FptA and FptX are involved in the ferri-PCH uptake, but this process requires increasing molecular and biochemical knowledge. Here, using bacterial two-hybrid and copurification assays we identified the fatty acid coenzyme-A ligase FadD1 as a novel interacting partner of the inner membrane transporter FptX and found that FadD1 may play a role in PCH production. We managed to purify the FadD1-FptX inner membrane complex and obtained low-resolution 3D models, opening the way for future high-resolution structures.

Keywords: Pseudomonas aeruginosa; coenzyme-A ligase; iron uptake; membrane complex; pyochelin siderophore.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Outer Membrane Proteins / chemistry*
  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Outer Membrane Proteins / metabolism
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Binding Sites
  • Cell Membrane / chemistry
  • Cell Membrane / metabolism
  • Cloning, Molecular
  • Coenzyme A Ligases / chemistry*
  • Coenzyme A Ligases / genetics
  • Coenzyme A Ligases / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Fatty Acids / chemistry
  • Fatty Acids / metabolism
  • Gene Expression
  • Iron / chemistry*
  • Iron / metabolism
  • Models, Molecular
  • Phenols / metabolism*
  • Plasmids / chemistry
  • Plasmids / metabolism
  • Protein Binding
  • Protein Conformation
  • Protein Interaction Domains and Motifs
  • Protein Isoforms / chemistry
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • Pseudomonas aeruginosa / genetics
  • Pseudomonas aeruginosa / metabolism*
  • Receptors, Cell Surface / chemistry*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Siderophores / biosynthesis
  • Thiazoles / metabolism*

Substances

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • FPTA protein, Pseudomonas
  • Fatty Acids
  • Phenols
  • Protein Isoforms
  • Receptors, Cell Surface
  • Recombinant Proteins
  • Siderophores
  • Thiazoles
  • pyochelin
  • Iron
  • Coenzyme A Ligases