Recreational Dance Practice Modulates Lymphocyte Profile and Function in Diabetic Women

Int J Sports Med. 2021 Jun;42(8):749-759. doi: 10.1055/a-1309-2037. Epub 2020 Dec 15.

Abstract

This study aimed to investigate the impact of a 16-week dance-based aerobic exercise program on lymphocyte function in healthy and type 2 diabetes mellitus (T2DM) women. We enrolled 23 women: 11 with T2DM and 12 non-diabetic controls. Initially, we performed anthropometry and body composition measurements, afterwards, plasma levels of C-reactive protein, lipids, and glucose were determined. We used flow cytometry to measure the CD25 and CD28 expression in circulating lymphocytes, T-regulatory (Treg) cell percentage, lymphocyte proliferation, and cytokines released by cultured lymphocytes. The T2DM group had a lower proportion of CD28+ cells and a higher percentage of Treg lymphocytes and proliferative capacity at the baseline compared with the control group. After 16 weeks of the program, differences in lymphocytes between the T2DM and the control groups disappeared. The dance program promoted IL-10 increase in both groups. We found decreased IL-4, IL-2, and IL-6 secretion in lymphocytes from the control group and increased IL-17 secretion and IL-10/IL-17 ratio in the T2DM group after the program. The program promoted marked changes in lymphocytes in diabetic women, leading to a balance between the different profiles.

MeSH terms

  • Aged
  • Blood Glucose / analysis
  • Body Composition
  • C-Reactive Protein / analysis
  • CD28 Antigens / blood*
  • Case-Control Studies
  • Cell Proliferation
  • Cytokines / metabolism
  • Dancing / physiology*
  • Diabetes Mellitus, Type 2 / blood*
  • Diabetes Mellitus, Type 2 / immunology
  • Exercise / physiology*
  • Female
  • Humans
  • Interleukin-2 Receptor alpha Subunit / blood*
  • Interleukins / blood
  • Lipids / blood
  • Lymphocytes / cytology
  • Lymphocytes / metabolism*
  • Lymphocytes / physiology
  • Middle Aged
  • T-Lymphocytes, Regulatory / cytology
  • T-Lymphocytes, Regulatory / metabolism
  • T-Lymphocytes, Regulatory / physiology
  • Time Factors

Substances

  • Blood Glucose
  • CD28 Antigens
  • Cytokines
  • IL2RA protein, human
  • Interleukin-2 Receptor alpha Subunit
  • Interleukins
  • Lipids
  • C-Reactive Protein