Influence of single and double immunomagnetic depletion on the hemopoietic capacity of marrow in patients with advanced neuroblastoma submitted to autologous bone marrow transplantation

Bone Marrow Transplant. 1987 Dec;2(4):413-9.

Abstract

Eighteen marrows from 16 patients with advanced neuroblastoma were submitted to a single or double immunomagnetic depletion procedure to purge them of tumor cells. Analysis of results showed that the CFU-GM recoveries were 59% +/- 16 (SE) and 33% +/- 16 after one or two cycles of incubation with microspheres. This led to yields of 7.8 x 10(4) and 4.1 x 10(4) CFU-GM available per kg body weight, respectively. Successful engraftment was demonstrated in all evaluable patients. However, some patients had delayed hematologic recoveries, particularly for platelets.

MeSH terms

  • Antibodies, Monoclonal / therapeutic use
  • Bone Marrow Cells
  • Bone Marrow Transplantation*
  • Cell Separation / methods
  • Colony-Forming Units Assay
  • Granulocytes
  • Hematopoiesis
  • Hematopoietic Stem Cells / cytology
  • Humans
  • Immunologic Techniques
  • Leukocytes, Mononuclear
  • Macrophages
  • Magnetics
  • Neuroblastoma / pathology
  • Neuroblastoma / therapy*
  • Transplantation, Autologous

Substances

  • Antibodies, Monoclonal