Discovery and Characterization of a Novel Ampelovirus on Firespike

Yaqin Wang; Yu Song; Yongzhi Wang; Mengji Cao; Tao Hu; Xueping Zhou

doi:10.3390/v12121452

Discovery and Characterization of a Novel Ampelovirus on Firespike

Viruses. 2020 Dec 16;12(12):1452. doi: 10.3390/v12121452.

Authors

Yaqin Wang¹, Yu Song¹, Yongzhi Wang^{1

2}, Mengji Cao³, Tao Hu¹, Xueping Zhou^{1

2}

Affiliations

¹ State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
² State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
³ National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, China.

Abstract

A novel RNA virus was identified in firespike (Odontonema tubaeforme) plants exhibiting leaf curling and chlorosis. The molecular features of the viral genomic RNA and proteins resemble those of ampeloviruses. Based on sequence comparisons and phylogenetic analysis, we propose a new species in the genus Ampelovirus, which we have tentatively named Firespike leafroll-associated virus (FLRaV). Bioassays showed that the virus is mechanically transmissible to Nicotiana benthamiana. In addition, a full-length cDNA clone of FLRaV could successfully infect N. benthamiana via agroinfiltration.

Keywords: Ampelovirus; Closteroviridae; firespike leafroll-associated virus; infectious cDNA clone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Closteroviridae / classification
Closteroviridae / genetics
Closteroviridae / isolation & purification*
Genome, Viral
High-Throughput Nucleotide Sequencing
Nicotiana / virology
Phylogeny
Plants / virology*