Afucosylated IgG characterizes enveloped viral responses and correlates with COVID-19 severity

Science. 2021 Feb 26;371(6532):eabc8378. doi: 10.1126/science.abc8378. Epub 2020 Dec 23.

Abstract

Immunoglobulin G (IgG) antibodies are crucial for protection against invading pathogens. A highly conserved N-linked glycan within the IgG-Fc tail, which is essential for IgG function, shows variable composition in humans. Afucosylated IgG variants are already used in anticancer therapeutic antibodies for their increased activity through Fc receptors (FcγRIIIa). Here, we report that afucosylated IgG (approximately 6% of total IgG in humans) are specifically formed against enveloped viruses but generally not against other antigens. This mediates stronger FcγRIIIa responses but also amplifies brewing cytokine storms and immune-mediated pathologies. Critically ill COVID-19 patients, but not those with mild symptoms, had high concentrations of afucosylated IgG antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), amplifying proinflammatory cytokine release and acute phase responses. Thus, antibody glycosylation plays a critical role in immune responses to enveloped viruses, including COVID-19.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antibodies, Viral / blood
  • Antibodies, Viral / chemistry
  • Antibodies, Viral / immunology*
  • COVID-19 / immunology*
  • COVID-19 / physiopathology
  • Cells, Cultured
  • Critical Illness
  • Cytomegalovirus / immunology
  • Female
  • Fucose / analysis
  • Glycosylation
  • HIV / immunology
  • Hepatitis B Vaccines / immunology
  • Humans
  • Immunoglobulin Fc Fragments / chemistry
  • Immunoglobulin Fc Fragments / immunology
  • Immunoglobulin G / blood
  • Immunoglobulin G / chemistry
  • Immunoglobulin G / immunology*
  • Inflammation
  • Interleukin-6 / biosynthesis
  • Interleukin-6 / immunology
  • Macrophages / immunology
  • Male
  • Middle Aged
  • Parvovirus B19, Human / immunology
  • SARS-CoV-2 / immunology*
  • Severity of Illness Index
  • Spike Glycoprotein, Coronavirus / immunology
  • Vaccines, Subunit / immunology
  • Young Adult

Substances

  • Antibodies, Viral
  • Hepatitis B Vaccines
  • IL6 protein, human
  • Immunoglobulin Fc Fragments
  • Immunoglobulin G
  • Interleukin-6
  • Spike Glycoprotein, Coronavirus
  • Vaccines, Subunit
  • spike protein, SARS-CoV-2
  • Fucose