On the NF-Y regulome as in ENCODE (2019)

PLoS Comput Biol. 2020 Dec 28;16(12):e1008488. doi: 10.1371/journal.pcbi.1008488. eCollection 2020 Dec.

Abstract

NF-Y is a trimeric Transcription Factor -TF- which binds with high selectivity to the conserved CCAAT element. Individual ChIP-seq analysis as well as ENCODE have progressively identified locations shared by other TFs. Here, we have analyzed data introduced by ENCODE over the last five years in K562, HeLa-S3 and GM12878, including several chromatin features, as well RNA-seq profiling of HeLa cells after NF-Y inactivation. We double the number of sequence-specific TFs and co-factors reported. We catalogue them in 4 classes based on co-association criteria, infer target genes categorizations, identify positional bias of binding sites and gene expression changes. Larger and novel co-associations emerge, specifically concerning subunits of repressive complexes as well as RNA-binding proteins. On the one hand, these data better define NF-Y association with single members of major classes of TFs, on the other, they suggest that it might have a wider role in the control of mRNA production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CCAAT-Binding Factor / metabolism*
  • Chromatin / metabolism
  • Chromatin Immunoprecipitation
  • Datasets as Topic
  • HeLa Cells
  • Humans
  • Sequence Analysis, RNA

Substances

  • CCAAT-Binding Factor
  • Chromatin

Grants and funding

R. M. received AIRC Grant 19050.The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.