Derivation of iPSC lines from three young healthy donors of Caucasian origin (NUIGi035-A; NUIGi036-A; NUIGi037-A)

Yicheng Ding; Berta Marcó de la Cruz; Veronica McInerney; Yin Lu; Guangming Yang; Xiaohong Qian; Weidong Li; Janusz Krawczyk; Linda Howard; Timothy O'Brien; Louise Gallagher; Sanbing Shen

doi:10.1016/j.scr.2020.102101

Derivation of iPSC lines from three young healthy donors of Caucasian origin (NUIGi035-A; NUIGi036-A; NUIGi037-A)

Stem Cell Res. 2020 Dec:49:102101. doi: 10.1016/j.scr.2020.102101. Epub 2020 Nov 27.

Authors

Affiliations

¹ Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI) Galway, Ireland.
² HRB Clinical Research Facility, National University of Ireland (NUI) Galway, Ireland.
³ School of Pharmacy, Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine (TCM) Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
⁴ College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
⁵ State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences, Beijing Institute of Proteomics, Beijing, China.
⁶ Bio-X Institutes, Key Laboratory for the Genetics of Development and Neuropsychiatric Disorders (Ministry of Education), Shanghai Key Laboratory of Psychotic Disorders, and Brain Science and Technology Research Center, Shanghai Jiao Tong University, Shanghai, China.
⁷ Department of Haematology, Galway University Hospital, Ireland.
⁸ Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI) Galway, Ireland; CÚRAM, National University of Ireland (NUI) Galway, Ireland.
⁹ Trinity Institute of Neuroscience, Trinity College Dublin, Dublin, Ireland. Electronic address: [email protected].
¹⁰ Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI) Galway, Ireland; FutureNeuro Research Centre, Royal College of Surgeons in Ireland, Dublin D02, Ireland. Electronic address: [email protected].

PMID: 33370872
DOI: 10.1016/j.scr.2020.102101

Abstract

The induced pluripotent stem cell (iPSC) technology has offered an unprecedented opportunity for disease modelling and drug discovery. Here we used non-integrating Sendai viral method and derived iPSCs from three young healthy Caucasian donors. All iPSCs expressed pluripotency markers highly and could be differentiated into three germ lineages. They possess normal karyotype which was confirmed by whole genome SNP array. The availability of the healthy control iPSCs offers an opportunity for phenotypic comparison and genome editing for a variety of diseases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cell Line*
Humans
Induced Pluripotent Stem Cells*
Sendai virus
White People