Generation of an IPSC line from a patient with hypertrophic cardiomyopathy carrying a mutation in MYH6 gene

Lu Wang; Xiaowei Li; Wanrong Fu; Shuai Zhang; Wenting Du; Jing Zhao; Mengduan Liu; Mengyu Wang; Yangyang Liu; Liang Hu; Zhongren Ding; Ling Li; Xiaoyan Zhao; Jianzeng Dong

doi:10.1016/j.scr.2020.102138

Generation of an IPSC line from a patient with hypertrophic cardiomyopathy carrying a mutation in MYH6 gene

Stem Cell Res. 2020 Dec 24:50:102138. doi: 10.1016/j.scr.2020.102138. Online ahead of print.

Authors

Lu Wang¹, Xiaowei Li¹, Wanrong Fu¹, Shuai Zhang², Wenting Du¹, Jing Zhao¹, Mengduan Liu¹, Mengyu Wang¹, Yangyang Liu¹, Liang Hu¹, Zhongren Ding¹, Ling Li³, Xiaoyan Zhao¹, Jianzeng Dong⁴

Affiliations

¹ Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Henan Key Laboratory of Hereditary Cardiovascular Diseases, Zhengzhou 450052, China.
² Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Centre for Cardiovascular Diseases, No. 2 Beijing Anzhen Road, Chaoyang District, Beijing 100029, China.
³ Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
⁴ Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Henan Key Laboratory of Hereditary Cardiovascular Diseases, Zhengzhou 450052, China; Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Centre for Cardiovascular Diseases, No. 2 Beijing Anzhen Road, Chaoyang District, Beijing 100029, China. Electronic address: [email protected].

PMID: 33385793
DOI: 10.1016/j.scr.2020.102138

Abstract

An induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMCs) of a 41-year-old male patient with hypertrophic cardiomyopathy who carries a G3755A heterozygote mutation in the MYH6 gene. The generated iPSC line expressed pluripotency markers, exhibited a normal karyotype, presented the specific mutation, and demonstrated differentiation potential into three germ layers in vitro.