N3 -Kethoxal-Based Bioorthogonal Intracellular RNA Labeling

Chembiochem. 2021 May 4;22(9):1559-1562. doi: 10.1002/cbic.202000755. Epub 2021 Feb 5.

Abstract

There is growing interest in developing intracellular RNA tools. Herein, we describe a strategy for N3 -kethoxal (N3 K)-based bioorthogonal intracellular RNA functionalization. With N3 K labeling followed by an in vivo click reaction with DBCO derivatives, RNA can be modified with fluorescent or phenol groups. This strategy provides a new way of labeling RNA inside cells.

Keywords: RNA; azides; bioorthogonal; intracellular; probes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascorbate Peroxidases / metabolism
  • Azides / chemistry
  • Butanones / chemistry*
  • Click Chemistry
  • Fluorescent Dyes / chemistry
  • HeLa Cells
  • Humans
  • Proteins / chemistry
  • Proteins / metabolism
  • RNA / chemistry*
  • RNA / metabolism

Substances

  • Azides
  • Butanones
  • Fluorescent Dyes
  • Proteins
  • RNA
  • kethoxal
  • Ascorbate Peroxidases