A dual-modal PET/near infrared fluorescent nanotag for long-term immune cell tracking

Biomaterials. 2021 Feb:269:120630. doi: 10.1016/j.biomaterials.2020.120630. Epub 2020 Dec 23.

Abstract

Adoptive cell transfer of targeted chimeric antigen receptor (CAR) T cells has emerged as a highly promising cancer therapy. The pharmacodynamic action or CAR T cells is closely related to their pharmacokinetic profile; because of this as well as the risk of non-specific action, it is important to monitor their biodistribution and fate following infusion. To this end, we developed a dual-modal PET/near infrared fluorescent (NIRF) nanoparticle-based imaging agent for non-genomic labeling of human CAR T cells. Since the PET/NIRF nanoparticles did not affect cell viability or cytotoxic functionality and enabled long-term whole-body CAR T cell tracking using PET and NIRF in an ovarian peritoneal carcinomatosis model, this platform is a viable imaging technology to be applied in other cancer models.

Keywords: Dual-modal; Immune cell labeling; Nanomedicine; Near-infrared fluorescence; PET; Tracking.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Tracking*
  • Humans
  • Immunotherapy, Adoptive*
  • Positron-Emission Tomography
  • Tissue Distribution