Use of Plasma Metagenomic Next-generation Sequencing for Pathogen Identification in Pediatric Endocarditis

Pediatr Infect Dis J. 2021 May 1;40(5):486-488. doi: 10.1097/INF.0000000000003038.

Abstract

Pediatric infective endocarditis incurs significant morbidity and generally occurs among children with underlying heart disease. Identification of a pathogen is critical in determining appropriate therapy. However, standard diagnostic testing has limited sensitivity. We describe a case series of children with infective endocarditis in whom plasma next-generation sequencing (Karius, Redwood, CA) identified an organism in 8 of 10 cases.

MeSH terms

  • Adolescent
  • California / epidemiology
  • Cell-Free Nucleic Acids / blood*
  • Child
  • Endocarditis / microbiology*
  • Female
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Male
  • Metagenome*
  • Metagenomics / methods
  • Plasma / microbiology*
  • Retrospective Studies
  • Sequence Analysis, DNA

Substances

  • Cell-Free Nucleic Acids