Comparative E-Cadherin Digital Expression Analysis in HPV and non-HPV Related Squamous Cell Carcinoma of the Oral Cavity

Anticancer Res. 2021 Jan;41(1):163-167. doi: 10.21873/anticanres.14761.

Abstract

Background/aim: Oral squamous cell carcinoma (OSCC) demonstrates aggressive biological behavior in subgroups of patients with specific molecular characteristics. Concerning metastatic potential, disruption of cell to cell adhesion is a critical event in epithelial malignancies including OSCC. Our aim was to investigate the role of E-Cadherin expression in OSCC patients as a valuable protein marker.

Materials and methods: Fifty (n=50) tissue sections derived from primary OSCCs were analyzed by implementing an immunohistochemistry (IHC) assay based on a proper anti-E-cadherin antibody. Digital image analysis was also implemented for an objective evaluation of the corresponding protein expression levels.

Results: E-cadherin altered expression (low to negative) was observed in 34/50 (68%) cases, whereas the rest (16/50-32%) demonstrated normal (high to moderate) expression. E-Cadherin abnormal expressionwas associated with the stage of the examined malignancies (p=0.023), whereas no significant correlations with grade, gender, smoking status or human papilloma virus (HPV) history were observed.

Conclusion: E-Cadherin down regulation is frequently observed in OSCC and is correlated to a progressively aggressive phenotype of the malignancy in the corresponding patients (advanced stage), but it seems that the impact of HPV persistent infection on these patients is not a critical parameter.

Keywords: Carcinoma; HPV; analysis; e-cadherin; immunohistochemistry; oral.

MeSH terms

  • Alphapapillomavirus*
  • Biomarkers, Tumor
  • Cadherins / genetics*
  • Cadherins / metabolism
  • Carcinoma, Squamous Cell / etiology*
  • Carcinoma, Squamous Cell / metabolism
  • Carcinoma, Squamous Cell / pathology
  • Disease Susceptibility
  • Female
  • Gene Expression*
  • Humans
  • Immunohistochemistry
  • Male
  • Mouth Neoplasms / etiology*
  • Mouth Neoplasms / metabolism
  • Mouth Neoplasms / pathology
  • Neoplasm Grading
  • Neoplasm Staging
  • Papillomavirus Infections / complications*
  • Papillomavirus Infections / virology

Substances

  • Biomarkers, Tumor
  • Cadherins