Cathelicidin protects mice from Rhabdomyolysis-induced Acute Kidney Injury

Int J Med Sci. 2021 Jan 1;18(4):883-890. doi: 10.7150/ijms.52397. eCollection 2021.

Abstract

Background: Cathelicidins are ancient and well-conserved antimicrobial peptides (AMPs) with intriguing immunomodulatory properties in both infectious and non-infectious inflammatory diseases. In addition to direct antimicrobial activity, cathelicidins also participate in several signaling pathways inducing both pro-inflammatory and anti-inflammatory effects. Acute kidney injury (AKI) is common in critically ill patients and is associated with high mortality and morbidity. Rhabdomyolysis is a major trigger of AKI. Objectives: Here, we investigated the role of cathelicidins in non-infectious Acute kidney Injury (AKI). Method: Using an experimental model of rhabdomyolysis, we induced AKI in wild-type and cathelicidin-related AMP knockout (CRAMP-/-) mice. Results: We previously demonstrated that CRAMP-/- mice, as opposed wild-type mice, are protected from AKI during sepsis induced by cecal ligation and puncture. Conversely, in the current study, we show that CRAMP-/- mice are more susceptible to the rhabdomyolysis model of AKI. A more in-depth investigation of wild-type and CRAMP-/- mice revealed important differences in the levels of several inflammatory mediators. Conclusion: Cathelicidins can induce a varied and even opposing repertoire of immune-inflammatory responses depending on the subjacent disease and the cellular context.

Keywords: acute kidney injury; antimicrobial peptide; cathelicidin; inflammation; innate immunity; rhabdomyolysis; sepsis.

MeSH terms

  • Acute Kidney Injury / immunology*
  • Acute Kidney Injury / pathology
  • Animals
  • Antimicrobial Cationic Peptides / genetics
  • Antimicrobial Cationic Peptides / metabolism*
  • Cathelicidins
  • Disease Models, Animal
  • Glycerol / administration & dosage
  • Glycerol / toxicity
  • Humans
  • Inflammation / immunology
  • Inflammation / pathology
  • Injections, Intramuscular
  • Kidney / immunology
  • Kidney / pathology
  • Male
  • Mice
  • Mice, Knockout
  • Rhabdomyolysis / chemically induced
  • Rhabdomyolysis / complications*
  • Rhabdomyolysis / immunology
  • Signal Transduction / immunology*

Substances

  • Antimicrobial Cationic Peptides
  • Glycerol
  • Cathelicidins