In Situ 3D Label-Free Contactless Bioprinting of Cells through Diamagnetophoresis

Abdel Rahman Abdel Fattah; Elvira Meleca; Sarah Mishriki; Alina Lelic; Fei Geng; Rakesh P Sahu; Suvojit Ghosh; Ishwar K Puri

doi:10.1021/acsbiomaterials.6b00614

In Situ 3D Label-Free Contactless Bioprinting of Cells through Diamagnetophoresis

ACS Biomater Sci Eng. 2016 Dec 12;2(12):2133-2138. doi: 10.1021/acsbiomaterials.6b00614. Epub 2016 Nov 8.

Authors

Abdel Rahman Abdel Fattah¹, Elvira Meleca², Sarah Mishriki³, Alina Lelic⁴, Fei Geng⁴, Rakesh P Sahu⁴, Suvojit Ghosh⁴, Ishwar K Puri⁴

Affiliations

¹ Department of Mechanical Engineering, §School of Biomedical Engineering, ⊥McMaster Immunology Research Center, and ∥Department of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, OntarioL8S 4L7, Canada.
² Department of Mechanical Engineering, School of Biomedical Engineering, ⊥McMaster Immunology Research Center, and ∥Department of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, OntarioL8S 4L7, Canada.
³ Department of Mechanical Engineering, School of Biomedical Engineering, McMaster Immunology Research Center, and ∥Department of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, OntarioL8S 4L7, Canada.
⁴ Department of Mechanical Engineering, School of Biomedical Engineering, McMaster Immunology Research Center, and Department of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, OntarioL8S 4L7, Canada.

PMID: 33465889
DOI: 10.1021/acsbiomaterials.6b00614

Abstract

Using whole blood, we demonstrate the first realization of a novel macroscale, contactless, label-free method to print in situ three-dimensional (3D) cell assemblies of different morphologies and sizes. This novel bioprinting method does not use nozzles that can contaminate the cell suspension, or to which cells can adhere. Instead, we utilize the intrinsic diamagnetic properties of whole blood cells to magnetically manipulate them in situ in a nontoxic paramagnetic medium, creating (a) rectangular bar, (b) three-pointed star, and (c) spheroids of varying sizes. We envision the technique to be transferable to other cell lines, with potential applications in tissue engineering and drug screening.

Keywords: 3D bioprinting; cell magnetophoresis; fluid recirculation; paramagnetism.