EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes

Zhihong Wu; Yu Wang; Wenjing Han; Kun Yang; Erhan Hai; Rong Ma; Zhengyang Di; Fangzheng Shang; Rui Su; Ruijun Wang; Zhiying Wang; Yanjun Zhang; Jinquan Li

doi:10.5194/aab-63-461-2020

EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes

Arch Anim Breed. 2020 Dec 10;63(2):461-470. doi: 10.5194/aab-63-461-2020. eCollection 2020.

Authors

Zhihong Wu^#¹, Yu Wang^#², Wenjing Han^#³, Kun Yang¹, Erhan Hai¹, Rong Ma¹, Zhengyang Di¹, Fangzheng Shang¹, Rui Su¹, Ruijun Wang¹, Zhiying Wang¹, Yanjun Zhang¹, Jinquan Li^{4

5

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Affiliations

¹ College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, China.
² College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, China.
³ College of Chemistry and Life Science, Chifeng University, Chifeng, Inner Mongolia Autonomous Region 024000, China.
⁴ Key Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot, Inner Mongolia Autonomous Region 010018, China.
⁵ Engineering Research Center for Goat Genetics and Breeding, Inner Mongolia Autonomous Region, Hohhot, Inner Mongolia Autonomous Region 010018, China.
⁶ Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, Inner Mongolia Autonomous Region 010018, China.

^# Contributed equally.

Abstract

This study is focused on the detection of ectodysplasin A (EDA) and ectodysplasin A receptor (EDAR) mRNA expression levels and protein positions in seven stages of cashmere goat fetus development (45, 55, 65, 75 95, 115, and 135 d), with the main goal of investigating the effect of EDA and EDAR on genes related to hair follicle development. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to measure EDA and EDAR expression levels in seven stages of cashmere goat fetus development. Immunohistochemistry (IHC) was used to locate EDA and EDAR in the critical stage of fetal hair follicle development (45-135 d). EDA and EDAR expression in fetal fibroblasts and epithelial cells was interfered with by short hairpin RNA (sh-RNA). The results indicated that EDA and EDAR were both expressed in the skin tissue in the seven cashmere goat embryo stages. Moreover, EDA and EDAR play an important role in the formation of embryonic placode (Pc). After interfering with EDA and EDAR, the expression of BMP2, BMP4, noggin, $β$ -catenin, TGF- $β$ 2, Wnt-10b, and NOTCH1 in fibroblasts and epithelial cells changed significantly. This study provides a theoretical and experimental basis for further studying the molecular regulation mechanism of hair follicle development.