Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia

Cuilan Hou; Wei Liu; Lijian Xie; Junmin Zheng; Xiaonan Chen; Xiaomin Sun; Yongwei Zhang; Meng Xu; Yun Li; Tingting Xiao

doi:10.1016/j.scr.2021.102183

Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia

Stem Cell Res. 2021 Mar:51:102183. doi: 10.1016/j.scr.2021.102183. Epub 2021 Jan 18.

Authors

Cuilan Hou¹, Wei Liu², Lijian Xie², Junmin Zheng², Xiaonan Chen³, Xiaomin Sun², Yongwei Zhang², Meng Xu², Yun Li⁴, Tingting Xiao⁵

Affiliations

¹ Department of Cardiology, Shanghai Children's Hospital, Shanghai Jiaotong University, 200062, China; NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology, Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200062, China.
² Department of Cardiology, Shanghai Children's Hospital, Shanghai Jiaotong University, 200062, China.
³ Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200062, China.
⁴ Department of Cardiology, Shanghai Children's Hospital, Shanghai Jiaotong University, 200062, China; NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology, Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200062, China. Electronic address: [email protected].
⁵ Department of Cardiology, Shanghai Children's Hospital, Shanghai Jiaotong University, 200062, China; NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology, Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200062, China. Electronic address: [email protected].

PMID: 33497884
DOI: 10.1016/j.scr.2021.102183

Abstract

CTNNA3, first reported in association with arrhythmogenic right ventricular cardiomyopathy in 2003, is an unique component of both desmosomes and adherens junctions. Using Sendaivirus-mediated reprogramming, we generated an induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells of a child with arrhythmia. The iPSCs exhibited stable amplification, expressed pluripotent markers, and differentiated spontaneously into three germ layers in vitro. Additionally, this iPSC line was found to maintain a normal karyotype and retain the pathogenic mutation in CTNNA3, facilitating a platform to study the disease mechanisms of arrhythmia and dysfunctions related to CTNNA3 mutations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arrhythmias, Cardiac / genetics
Arrhythmogenic Right Ventricular Dysplasia*
Cell Differentiation
Child
China
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear