Abstract
Coronaviruses of bats and pangolins have been implicated in the origin and evolution of the pandemic SARS-CoV-2. We show that spikes from Guangdong Pangolin-CoVs, closely related to SARS-CoV-2, bind strongly to human and pangolin ACE2 receptors. We also report the cryo-EM structure of a Pangolin-CoV spike protein and show it adopts a fully-closed conformation and that, aside from the Receptor-Binding Domain, it resembles the spike of a bat coronavirus RaTG13 more than that of SARS-CoV-2.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Angiotensin-Converting Enzyme 2 / metabolism
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Animals
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Binding, Competitive
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COVID-19 / epidemiology
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COVID-19 / prevention & control*
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COVID-19 / virology
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Cryoelectron Microscopy
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Evolution, Molecular*
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Humans
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Models, Molecular
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Pandemics
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Pangolins / virology
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Protein Binding
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Protein Domains
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SARS-CoV-2 / genetics*
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SARS-CoV-2 / metabolism
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SARS-CoV-2 / physiology
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Spike Glycoprotein, Coronavirus / chemistry
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Spike Glycoprotein, Coronavirus / genetics*
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Spike Glycoprotein, Coronavirus / metabolism
Substances
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Spike Glycoprotein, Coronavirus
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spike protein, SARS-CoV-2
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ACE2 protein, human
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Angiotensin-Converting Enzyme 2