A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster

Takuya Hashimoto; Junko Hashimoto; Noritaka Kagaya; Takehiro Nishimura; Hikaru Suenaga; Makoto Nishiyama; Tomohisa Kuzuyama; Kazuo Shin-Ya

doi:10.1038/s41429-021-00410-9

A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster

J Antibiot (Tokyo). 2021 May;74(5):354-358. doi: 10.1038/s41429-021-00410-9. Epub 2021 Feb 8.

Authors

Takuya Hashimoto¹, Junko Hashimoto², Noritaka Kagaya¹, Takehiro Nishimura³, Hikaru Suenaga¹, Makoto Nishiyama^{4

5}, Tomohisa Kuzuyama^{5

6}, Kazuo Shin-Ya^{7

8}

Affiliations

¹ National Institute of Advanced Industrial Science and Technology, Tokyo, Japan.
² Japan Biological Informatics Consortium (JBIC), Tokyo, Japan.
³ Technology Research Association for Next Generation Natural Products Chemistry, Tokyo, Japan.
⁴ Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
⁵ Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan.
⁶ Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
⁷ National Institute of Advanced Industrial Science and Technology, Tokyo, Japan. [email protected].
⁸ Biotechnology Research Center, The University of Tokyo, Tokyo, Japan. [email protected].

PMID: 33558648
DOI: 10.1038/s41429-021-00410-9

Abstract

Using genome mining approach, we identified a novel biosynthetic gene cluster containing trans-AT type PKS genes from Streptomyces versipellis 4083-SVS6. A bacterial artificial chromosome (BAC) clone, pKU503JL68_PN1_P10-C12, accommodating the entire biosynthetic gene cluster was obtained from a BAC library. Heterologous expression of the biosynthetic gene cluster in Streptomyces lividans TK23 led to the production of a novel polyene compound, JBIR-159. We report herein the biosynthetic gene cluster for JBIR-159, and the heterologous expression, isolation, structure determination and a brief biological activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes, Artificial, Bacterial
Cloning, Molecular
Gene Expression Regulation, Bacterial
Streptomyces / metabolism*

Supplementary concepts

Streptomyces albus
Streptomyces versipellis