Machine intelligence identifies soluble TNFa as a therapeutic target for spinal cord injury

Sci Rep. 2021 Feb 9;11(1):3442. doi: 10.1038/s41598-021-82951-5.

Abstract

Traumatic spinal cord injury (SCI) produces a complex syndrome that is expressed across multiple endpoints ranging from molecular and cellular changes to functional behavioral deficits. Effective therapeutic strategies for CNS injury are therefore likely to manifest multi-factorial effects across a broad range of biological and functional outcome measures. Thus, multivariate analytic approaches are needed to capture the linkage between biological and neurobehavioral outcomes. Injury-induced neuroinflammation (NI) presents a particularly challenging therapeutic target, since NI is involved in both degeneration and repair. Here, we used big-data integration and large-scale analytics to examine a large dataset of preclinical efficacy tests combining five different blinded, fully counter-balanced treatment trials for different acute anti-inflammatory treatments for cervical spinal cord injury in rats. Multi-dimensional discovery, using topological data analysis (TDA) and principal components analysis (PCA) revealed that only one showed consistent multidimensional syndromic benefit: intrathecal application of recombinant soluble TNFα receptor 1 (sTNFR1), which showed an inverse-U dose response efficacy. Using the optimal acute dose, we showed that clinically-relevant 90 min delayed treatment profoundly affected multiple biological indices of NI in the first 48 h after injury, including reduction in pro-inflammatory cytokines and gene expression of a coherent complex of acute inflammatory mediators and receptors. Further, a 90 min delayed bolus dose of sTNFR1 reduced the expression of NI markers in the chronic perilesional spinal cord, and consistently improved neurological function over 6 weeks post SCI. These results provide validation of a novel strategy for precision preclinical drug discovery that is likely to improve translation in the difficult landscape of CNS trauma, and confirm the importance of TNFα signaling as a therapeutic target.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Artificial Intelligence*
  • Disease Models, Animal
  • Female
  • Injections, Spinal
  • Models, Neurological*
  • Rats
  • Rats, Long-Evans
  • Receptors, Tumor Necrosis Factor, Type I / pharmacology
  • Recombinant Proteins / pharmacology
  • Spinal Cord Injuries / drug therapy
  • Spinal Cord Injuries / metabolism*
  • Spinal Cord Injuries / pathology
  • Tumor Necrosis Factor-alpha / metabolism*

Substances

  • Receptors, Tumor Necrosis Factor, Type I
  • Recombinant Proteins
  • Tnfrsf1a protein, rat
  • Tumor Necrosis Factor-alpha