Generation of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locus

Benjamin Schmid; Bjørn Holst; Ulla Poulsen; Ida Jørring; Christian Clausen; M Rasmussen; Ulrike A Mau-Holzmann; Rachel Steeg; Hugh Nuthall; A Ebneth; A Cabrera-Socorro

doi:10.1016/j.scr.2021.102240

Generation of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locus

Stem Cell Res. 2021 Apr:52:102240. doi: 10.1016/j.scr.2021.102240. Epub 2021 Feb 11.

Authors

Affiliations

¹ Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.
² Institute of Medical Genetics and Applied Genomics, Division of Cytogenetics, Calwerstrasse 7, University of Tübingen, 72076, Germany.
³ Fraunhofer UK Research Ltd, Glasgow, UK.
⁴ Eli Lilly & Company Limited, Bracknell, UK.
⁵ Neuroscience Therapeutic Area, Janssen Research & Development, A Division of Janssen Pharmaceutica NV, UK.
⁶ Neuroscience Therapeutic Area, Janssen Research & Development, A Division of Janssen Pharmaceutica NV, UK. Electronic address: [email protected].

PMID: 33610017
DOI: 10.1016/j.scr.2021.102240

Abstract

Neurog2 is the gene encoding the neuronal transcription factor NGN2, which can convert stem cells into functional neurons in a fast and efficient way. Here we report the generation of two iPS cell lines, where DOX inducible constructs of neurog2 either without or with T2A-eGFP were inserted into the safe-site locus AAVS1. These iPS cell lines, BIONi010-C-13 and BIONi010-C-15, respectively, stay pluripotent without DOX but differentiate to (GFP positive) neurons when DOX is added without the need of differentiation factors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Gene Editing
Genes, Reporter
Induced Pluripotent Stem Cells*
Transgenes