Cloning and expression of the ChGstα and ChGstκ genes in the gills of Crassostrea hongkongensis under nanoparticulate and ionic Zn stress

Nanoparticulate and ionic Zn have potential impacts on the detoxification systems of organisms, and Gst genes play key roles in the detoxification of xenobiotics. In this study, we cloned the ChGstα and ChGstκ genes of C. hongkongensis, and studied their expression in gills under nanoparticulate and ionic Zn stress. The results showed that the coding sequences of the ChGstα and ChGstκ genes were 684 and 675 bp, respectively, and had no signal peptide; ChGstα was cytoplasmic, while ChGstκ was mitochondrial. The two genes were expressed in all 8 tested samples, with the most abundant expression observed in hemocytes for ChGstα and digestive glands for ChGstκ. After ZnCl₂ or ZnoNP challenge, the expression of ChGstα decreased significantly in the ZnCl₂ groups, and its expression was higher in the ZnoNP groups than in the ZnCl₂ groups. The expression of ChGstκ was significantly decreased in the ZnCl₂ and ZnoNP groups, and its expression was higher in the ZnoNP groups than in the ZnCl₂ groups except at 3 h post metal Zn stress, which suggested that ChGstα and ChGstκ were more sensitive to ZnoNP than ZnCl₂.