Micronodular thymic carcinoma with lymphoid hyperplasia: relevance of immunohistochemistry with a small panel of antibodies for diagnosis-a RYTHMIC study

Virchows Arch. 2021 Oct;479(4):741-746. doi: 10.1007/s00428-021-03044-2. Epub 2021 Feb 24.

Abstract

Micronodular thymic carcinoma with lymphoid hyperplasia (MNTCLH) is a rare form of thymic carcinoma. We present the experience of RYTHMIC, the French national network devoted to the treatment of thymic epithelial tumors through multidisciplinary tumor boards with a review of all tumors by pathologists for classification and staging. Six cases of MNTCLH were diagnosed during a review of 1007 thymic epithelial tumors. Histologically, epithelial cells with atypia and mitoses formed micronodules that were surrounded by an abundant lymphoid background with follicles. There was neither obvious fibro-inflammatory stroma nor necrosis. Spindle cells areas were common. Initial diagnosis was micronodular thymoma in two cases, cellular atypia being overlooked, eclipsed by the micronodular pattern. Immunohistochemistry with a panel of five antibodies showed that cytokeratins (AE1-AE3) and p63-positive epithelial cells also expressed CD5 and that there was no TdT-positive cells within the tumors. CD20 highlighted the lymphoid hyperplasia. Additionally epithelial cells also expressed CD117 and diffusely Glut 1. Twenty-seven micronodular thymomas with lymphoid stroma diagnosed during the same period did not show the CD5 and CD117 positivities seen in MNTCLH and contained TdT-positive lymphocytes. Three of the 6 patients with MNTCLH had adjuvant radiotherapy. Three patients with follow-up information were alive without recurrence at 38, 51, and 95 months. Our study shows that immunohistochemistry, such as that used in the RYTHMIC network with a small panel of antibodies, may easily help to confirm the correct diagnosis of MNTCLH, a rare and low-aggressive form of thymic carcinoma, and avoid the misdiagnosis of micronodular thymoma.

Keywords: Immunohistochemistry; Micronodular thymic carcinoma with lymphoid hyperplasia; Thymic neoplasm.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Antibodies
  • B-Lymphocytes / pathology
  • Carcinoma / pathology
  • Epithelial Cells / pathology
  • Female
  • Humans
  • Hyperplasia / metabolism
  • Hyperplasia / pathology*
  • Immunohistochemistry / methods
  • Lymphatic Diseases / pathology
  • Male
  • Middle Aged
  • Neoplasm Recurrence, Local / pathology
  • Retrospective Studies
  • Thymoma / diagnosis*
  • Thymoma / metabolism
  • Thymoma / pathology*

Substances

  • Antibodies