Topography and stoichiometry of acidic phosphoproteins in rat liver 60 S ribosomal subunit

FEBS Lett. 1988 May 9;232(1):29-34. doi: 10.1016/0014-5793(88)80380-6.

Abstract

In reconstitution experiments of active 60 S subunits from inactive core particles obtained by using dimethyl maleic anhydride (DMMA), we observed that the phosphoproteins P1-P2 were extracted from the subunit by DMMA as a complex with other proteins. This complex was separated by electrophoresis and zonal centrifugation and shown, after 125I iodination of its components, to contain L22 and S12 in addition to P1-P2. Results suggest that it contains two copies of P1-P2 for one of L22 and S12.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Centrifugation, Zonal
  • Electrophoresis, Polyacrylamide Gel
  • Liver / chemistry*
  • Maleic Anhydrides
  • Phosphoproteins / analysis*
  • RNA-Binding Proteins*
  • Rats
  • Ribosomal Proteins / analysis
  • Ribosomes / chemistry*

Substances

  • Maleic Anhydrides
  • Phosphoproteins
  • RNA-Binding Proteins
  • Ribosomal Proteins
  • Rpl22 protein, rat
  • phosphoprotein P2, ribosomal
  • ribosomal phosphoprotein P1
  • ribosomal protein S12
  • 2,3-dimethylmaleic anhydride