Relationship between Physical Activity, Oxidative Stress, and Total Plasma Antioxidant Capacity in Spanish Children from the GENOBOX Study

Francisco Jesús Llorente-Cantarero; Francisco Javier Aguilar-Gómez; Rosaura Leis; Gloria Bueno; Azahara I Rupérez; Augusto Anguita-Ruiz; Rocío Vázquez-Cobela; María Dolores Mesa; Luis A Moreno; Ángel Gil; Concepción María Aguilera; Mercedes Gil-Campos

doi:10.3390/antiox10020320

Relationship between Physical Activity, Oxidative Stress, and Total Plasma Antioxidant Capacity in Spanish Children from the GENOBOX Study

Antioxidants (Basel). 2021 Feb 20;10(2):320. doi: 10.3390/antiox10020320.

Authors

Francisco Jesús Llorente-Cantarero^{1

2}, Francisco Javier Aguilar-Gómez³, Rosaura Leis^{2

4}, Gloria Bueno^{2

5

6}, Azahara I Rupérez⁶, Augusto Anguita-Ruiz^{2

7

8}, Rocío Vázquez-Cobela^{2

4}, María Dolores Mesa^{7

8}, Luis A Moreno^{2

6}, Ángel Gil^{2

7

8}, Concepción María Aguilera^{2

7

8}, Mercedes Gil-Campos^{2

3}

Affiliations

¹ Department of Specific Didactics, Faculty of Education, Maimónides Institute of Biomedicine Research of Córdoba (IMIBIC), University of Córdoba, 14071 Córdoba, Spain.
² CIBEROBN, (Physiopathology of Obesity and Nutrition) Institute of Health Carlos III (ISCIII), 28029 Madrid, Spain.
³ Metabolism and Investigation Unit, Reina Sofía University Hospital, Maimónides Institute of Biomedicine Research of Córdoba (IMIBIC), University of Córdoba, 14004 Córdoba, Spain.
⁴ Unit of Investigation in Nutrition, Growth and Human Development of Galicia, Pediatric Department, University of Santiago de Compostela, Clinic University Hospital of Santiago, Instituto de Investigación Sanitaria de Santiago (IDIS), 15706 Santiago de Compostela, Spain.
⁵ Pediatric Endocrinology Unit, Clinic University Hospital Lozano Blesa, Facultad de Medicina, Universidad de Zaragoza, 50009 Zaragoza, Spain.
⁶ GENUD Research Group, Instituto Agroalimentario de Aragón (IA2), Instituto de Investigación Sanitaria (IIS) Aragón, University of Zaragoza, 50009 Zaragoza, Spain.
⁷ Department of Biochemistry and Molecular Biology II, Institute of Nutrition and Food Technology "José Mataix", Center of Biomedical Research, University of Granada, Armilla, 18016 Granada, Spain.
⁸ Instituto de Investigación Biosanitaria ibs Granada, 18014 Granada, Spain.

Abstract

The World Health Organization has recommended performing at least 60 min a day of moderate-to-vigorous physical activity (MVPA) and reducing sedentarism in children and adolescents to offer significant health benefits and mitigate health risks. Physical fitness and sports practice seem to improve oxidative stress (OS) status during childhood. However, to our knowledge, there are no data regarding the influence of objectively-measured physical activity (PA) and sedentarism on OS status in children and adolescents. The present study aimed to evaluate the influence of moderate and vigorous PA and sedentarism on OS and plasma total antioxidant capacity (TAC) in a selected Spanish population of 216 children and adolescents from the GENOBOX study. PA (light, moderate, and vigorous) and sedentarism (i.e., sedentary time (ST)) were measured by accelerometry. A Physical Activity-Sedentarism Score (PASS) was developed integrating moderate and vigorous PA and ST levels. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) and isoprostane F2α (F₂-IsoPs), as markers of OS, were determined by ELISA; and TAC was estimated by colorimetry using an antioxidant kit. A higher PASS was associated with lower plasma TAC and urinary 8-OHdG and F₂-IsoPs, showing a better redox profile. Reduced OS markers (8-OHdG and F₂-IsoPs) in children with higher PASS may diminish the need of maintaining high concentrations of antioxidants in plasma during rest to achieve redox homeostasis.

Keywords: 8-hydroxy-2′-deoxyguanosine; accelerometry; isoprostane F2α; oxidative stress; physical activity; plasma total antioxidant capacity.