Dipylidium caninum draft genome - a new resource for comparative genomic and genetic explorations of flatworms

Guo-Hua Liu; Pasi K Korhonen; Neil D Young; Jiang Lu; Tao Wang; Yi-Tian Fu; Anson V Koehler; Andreas Hofmann; Bill C H Chang; Shuai Wang; Nan Li; Chu-Yu Lin; Hui Zhang; Lingzi Xiangli; Lin Lin; Wei-Min Liu; Nan Li; Hua-Wei Li; Robin B Gasser; Xing-Quan Zhu

doi:10.1016/j.ygeno.2021.02.019

Dipylidium caninum draft genome - a new resource for comparative genomic and genetic explorations of flatworms

Genomics. 2021 May;113(3):1272-1280. doi: 10.1016/j.ygeno.2021.02.019. Epub 2021 Mar 4.

Authors

Guo-Hua Liu¹, Pasi K Korhonen², Neil D Young², Jiang Lu³, Tao Wang², Yi-Tian Fu⁴, Anson V Koehler², Andreas Hofmann⁵, Bill C H Chang², Shuai Wang⁶, Nan Li³, Chu-Yu Lin³, Hui Zhang³, Lingzi Xiangli³, Lin Lin³, Wei-Min Liu³, Nan Li³, Hua-Wei Li³, Robin B Gasser⁷, Xing-Quan Zhu⁸

Affiliations

¹ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China; College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. Electronic address: [email protected].
² Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville 3010, Australia.
³ Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China; Shenzhen Zhong Nong Jing Yue Biotech Company Limited, Shenzhen 518124, China.
⁴ College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
⁵ Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville 3010, Australia; Griffith Institute for Drug Discovery, Griffith University, Dathan 4111, Australia.
⁶ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
⁷ Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville 3010, Australia. Electronic address: [email protected].
⁸ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China; College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China. Electronic address: [email protected].

PMID: 33677058
DOI: 10.1016/j.ygeno.2021.02.019

Abstract

Here, we present a draft genome of the tapeworm Dipylidium caninum (family Dipylidiidae) and compare it with other cestode genomes. This draft genome of D. caninum is 110 Mb in size, has a repeat content of ~13.4% and is predicted to encode ~10,000 protein-coding genes. We inferred excretory/secretory molecules (representing the secretome), other key groups of proteins (including peptidases, kinases, phosphatases, GTPases, receptors, transporters and ion-channels) and predicted potential intervention targets for future evaluation. Using 144 shared single-copy orthologous sequences, we investigated the genetic relationships of cestodes for which nuclear genomes are available. This study provides first insights into the molecular biology of D. caninum and a new resource for comparative genomic and genetic explorations of this and other flatworms.

Keywords: Cestode; Comparative genomic and genetic analyses; Dipylidium caninum; Nuclear genome; Tapeworm.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cestoda* / genetics
Cestode Infections*
Genomics
Platyhelminths*