Introduction: Triatomines are insect vectors of Trypanosoma cruzi, the etiological agent of Chagas disease.
Methods: Triatomines were collected from households and by dissecting palm trees in the peri-urban areas of Cruzeiro do Sul (Acre); they were identified using a specific key and via genital analyses. Trypanosomatid infection was determined through microscopy and polymerase chain reaction.
Results: In total, 116 triatomines of the species Eratyrus mucronatus, Rhodnius pictipes, R. stali, and R. montenegrensis were collected, of which 13.8% were positive for T. cruzi.
Conclusions: Four species of triatomines presented an infection rate above 13% in the Boca do Moa community.