Immunotyping and Quantification of Melanoma Tumor-Infiltrating Lymphocytes

Max O Meneveau; Zeyad T Sahli; Kevin T Lynch; Ileana S Mauldin; Craig L Slingluff Jr

doi:10.1007/978-1-0716-1205-7_36

Immunotyping and Quantification of Melanoma Tumor-Infiltrating Lymphocytes

Methods Mol Biol. 2021:2265:515-528. doi: 10.1007/978-1-0716-1205-7_36.

Authors

Max O Meneveau¹, Zeyad T Sahli¹, Kevin T Lynch¹, Ileana S Mauldin¹, Craig L Slingluff Jr²

Affiliations

¹ Department of Surgery, The University of Virginia Health System, Charlottesville, VA, USA.
² Department of Surgery, The University of Virginia Health System, Charlottesville, VA, USA. [email protected].

PMID: 33704737
DOI: 10.1007/978-1-0716-1205-7_36

Abstract

The density of tumour-infiltrating lymphocytes (TILs) in melanoma is correlated with improved clinical prognosis; however, standardized TIL immunotyping and quantification protocols are lacking. Herein, we provide a review of the technologies being utilized for the immunotyping and quantification of melanoma TILs.

Keywords: Immunofluorescence histology; Immunotyping; Melanoma; Quantification; Time of flight mass cytometry; Tumor-infiltrating lymphocyte.

MeSH terms

Humans
Immunophenotyping*
Lymphocytes, Tumor-Infiltrating* / immunology
Lymphocytes, Tumor-Infiltrating* / pathology
Melanoma* / immunology
Melanoma* / pathology