Cytokine gene polymorphisms are associated with response to blinatumomab in B-cell acute lymphoblastic leukemia

Eur J Haematol. 2021 Jun;106(6):851-858. doi: 10.1111/ejh.13622. Epub 2021 Mar 24.

Abstract

Blinatumomab is a bispecific T cell-engaging antibody approved for treatment of relapsed/refractory (r/r) ALL, with 40%-50% complete response (CR)/CR with incomplete count recovery (CRi). Cytokine release syndrome (CRS) as a major adverse effect after blinatumomab therapy. Here, we evaluated the possible association between single-nucleotide polymorphisms (SNPs) in cytokine genes, disease response, and CRS in r/r ALL patients who received blinatumomab between 2012 and 2017 at our center (n = 66), using patients' archived DNA samples. With a median duration of 9.5 months (range: 1-37), 37 patients (56.1%) achieved CR/CRi, 54 (81.8%) experienced CRS (G1: n = 35, G2: n = 14, G3: n = 5), and 9 (13.6%) developed neurotoxicity. By multivariable analysis, after adjusting for high disease burden, one SNP on IL2 (rs2069762), odds ratio (OR) = 0.074 (95% CI: NE-0.43, P = .01) and one SNP on IL17A (rs4711998), OR = 0.28 (95% CI: 0.078-0.92, P = .034) were independently associated with CR/CRi. None of the analyzed SNPs were associated with CRS. To our knowledge, this is the first study demonstrating a possible association between treatment response to blinatumomab and SNPs. Our hypothesis-generated data suggest a potential role for IL-17 and IL-2 in blinatumomab response and justify a larger confirmatory study, which may lead to personalized blinatumomab immunotherapy for B-ALL.

Keywords: acute lymphoblastic leukemia; blinatumomab; cytokine gene polymorphism.

Publication types

  • Clinical Trial

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Antibodies, Bispecific* / administration & dosage
  • Antibodies, Bispecific* / adverse effects
  • Child
  • Cytokine Release Syndrome* / blood
  • Cytokine Release Syndrome* / chemically induced
  • Cytokine Release Syndrome* / genetics
  • Female
  • Humans
  • Interleukin-17* / blood
  • Interleukin-17* / genetics
  • Interleukin-2* / blood
  • Interleukin-2* / genetics
  • Male
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma* / blood
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma* / drug therapy
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma* / genetics
  • Retrospective Studies

Substances

  • Antibodies, Bispecific
  • IL2 protein, human
  • Il17a protein, mouse
  • Interleukin-17
  • Interleukin-2
  • blinatumomab