Bardet-Biedl syndrome-7 (BBS7) shows treatment potential and a cone-rod dystrophy phenotype that recapitulates the non-human primate model

Ophthalmic Genet. 2021 Jun;42(3):252-265. doi: 10.1080/13816810.2021.1888132. Epub 2021 Mar 17.

Abstract

Purpose: To provide a detailed ophthalmic phenotype of two male patients with Bardet-Biedl Syndrome (BBS) due to mutations in the BBS7 geneMethods: Two brothers ages 26 (Patient 1, P1) and 23 (P2) underwent comprehensive ophthalmic evaluations over three years. Visual function was assessed with full-field electroretinograms (ffERGs), kinetic and chromatic perimetry, multimodal imaging with spectral domain optical coherence tomography (SD-OCT), fundus autofluorescence (FAF) with short- (SW) and near-infrared (NIR) excitation lights and adaptive optics scanning light ophthalmoscopy (AOSLO).Results: Both siblings had a history of obesity and postaxial polydactyly; P2 had diagnoses of type 1 Diabetes Mellitus, Addison's disease, high-functioning autism-spectrum disorder and -12D myopia. Visual acuities were better than 20/30. Kinetic fields were moderately constricted. Cone-mediated ffERGs were undetectable, rod ERGs were ~80% of normal mean. Static perimetry showed severe central cone and rod dysfunction. Foveal to parafoveal hypoautofluorescence, most obvious on NIR-FAF, co-localized with outer segment shortening/loss and outer nuclear layer thinning by SD-OCT, and with reduced photoreceptors densities by AOSLO. A structural-functional dissociation was confirmed for cone- and rod-mediated parameters. Worsening of the above abnormalities was documented by SD-OCT and FAF in P2 at 3 years. Gene screening identified compound heterozygous mutations in BBS7 (p.Val266Glu: c.797 T > A of maternal origin; c.1781_1783delCAT, paternal) in both patients.Conclusions: BBS7-associated retinal degeneration may present as a progressive cone-rod dystrophy pattern, reminiscent of both the murine and non-human primate models of the disease. Predominantly central retinal abnormalities in both cone and rod photoreceptors showed a structural-functional dissociation, an ideal scenario for gene augmentation treatments.

Keywords: BBS; BBS7; Bardet-Biedl; OCT; RP; adaptive optics ophthalmoscopy; cone dystrophy; cone-rod dystrophy; retinitis pigmentosa.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics*
  • Adult
  • Bardet-Biedl Syndrome / diagnostic imaging
  • Bardet-Biedl Syndrome / genetics*
  • Bardet-Biedl Syndrome / physiopathology
  • Cone-Rod Dystrophies / diagnostic imaging
  • Cone-Rod Dystrophies / genetics*
  • Cone-Rod Dystrophies / physiopathology
  • Cytoskeletal Proteins / genetics*
  • Electroretinography
  • Genetic Therapy
  • Humans
  • Male
  • Models, Animal
  • Mutation / genetics
  • Ophthalmoscopy
  • Optical Imaging
  • Phenotype
  • Retina / physiopathology
  • Siblings
  • Tomography, Optical Coherence
  • Visual Acuity
  • Visual Field Tests
  • Young Adult

Substances

  • Adaptor Proteins, Signal Transducing
  • Bbs7 protein, human
  • Cytoskeletal Proteins

Supplementary concepts

  • Bardet-Biedl Syndrome 7