TISIGNER.com: web services for improving recombinant protein production

Bikash K Bhandari; Chun Shen Lim; Paul P Gardner

doi:10.1093/nar/gkab175

TISIGNER.com: web services for improving recombinant protein production

Nucleic Acids Res. 2021 Jul 2;49(W1):W654-W661. doi: 10.1093/nar/gkab175.

Authors

Bikash K Bhandari¹, Chun Shen Lim¹, Paul P Gardner^{1

2}

Affiliations

¹ Department of Biochemistry, School of Biomedical Sciences, University of Otago, Dunedin 9054, New Zealand.
² Biomolecular Interaction Centre, University of Canterbury, Christchurch 8140, New Zealand.

Abstract

Experiments that are planned using accurate prediction algorithms will mitigate failures in recombinant protein production. We have developed TISIGNER (https://tisigner.com) with the aim of addressing technical challenges to recombinant protein production. We offer three web services, TIsigner (Translation Initiation coding region designer), SoDoPE (Soluble Domain for Protein Expression) and Razor, which are specialised in synonymous optimisation of recombinant protein expression, solubility and signal peptide analysis, respectively. Importantly, TIsigner, SoDoPE and Razor are linked, which allows users to switch between the tools when optimising genes of interest.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Internet
Peptide Chain Initiation, Translational
Protein Sorting Signals
Recombinant Proteins / biosynthesis*
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Software*
Solubility

Substances

Protein Sorting Signals
Recombinant Proteins