Purpose: To synthetise mouse parathyroid hormone related protein 1-36 (PTHrP1-36) and to study its role in promoting alveolar bone formation.
Methods: pGEX-2TK/ PTHrP1-36 was constructed and PTHrP1-36 was expressed. The role of PTHrP1-36 in promoting alveolar bone formation was observed by treating bone marrow cells in vitro and ovariectomy-induced maxillofacial osteoporosis in vivo. Statistical analysis was performed using SPSS 10.0 software package.
Results: The results of PCR analysis showed that pGEX-2TK/ PTHrP1-36 plasmid was correct templates for expressing PTHrP1-36. The molecular weights obtained by SDS-PAGE were the same as the theoretical value after IPTG induction. Scale up production was carried out by 3-hour-induction with 1mM IPTG which was the optimal induction factors. Bone marrow cell culture revealed that synthetic PTHrP1-36 increased the number of total fibroblastic colony forming units (CFU-f), ALP positive CFU-f and calcific nodules. After PTHrP1-36 was subcutaneously administered to ovariectomy mice, density and volume of alveolar bone were improved, as showed by X-ray and histochemistry staining of collagen-l and H-E staining.
Conclusions: Synthetic PTHrP1-36 can promote bone marrow cell osteogenic differentiation and increase alveolar bone formation.