Prevalence of bla_KPC-2, bla_KPC-3 and bla_KPC-30-Carrying Plasmids in Klebsiella pneumoniae Isolated in a Brazilian Hospital

Klebsiella pneumoniae carbapenemase (KPC) actively hydrolyzes carbapenems, antibiotics often used a last-line treatment for multidrug-resistant bacteria. KPC clinical relevance resides in its widespread dissemination. In this work, we report the genomic context of KPC coding genes bla_KPC-2, bla_KPC-3 and bla_KPC-30 in multidrug-resistant Klebsiellapneumoniae isolates from Brazil. Plasmids harboring bla_KPC-3 and bla_KPC-30 were identified. Fifteen additional carbapenem-resistant K. pneumoniae isolates were selected from the same tertiary hospital, collected over a period of 8 years. Their genomes were sequenced in order to evaluate the prevalence and dissemination of bla_KPC-harboring plasmids. We found that bla_KPC genes were mostly carried by one of two isoforms of transposon Tn4401 (Tn4401a or Tn4401b) that were predominantly located on plasmids highly similar to the previously described plasmid pKPC_FCF3SP (IncN). The identified pKPC_FCF3SP-like plasmids carried either bla_KPC-2 or bla_KPC-30. Two K. pneumoniae isolates harbored pKpQIL-like (IncFII) plasmids, only recently identified in Brazil; one of them harbored bla_KPC-3 in a Tn4401a transposon. Underlining the risk of horizontal spread of KPC coding genes, this study reports the prevalence of bla_KPC-2 and the recent spread of bla_KPC-3, and bla_KPC-30, in association with different isoforms of Tn4401, together with high synteny of plasmid backbones among isolates studied here and in comparison with previous reports.