Molecular cloning and immune characterization of CIITA in artificially challenged Chinese tongue sole (Cynoglossus semilaevis) with Vibrio harveyi

Dev Comp Immunol. 2021 Aug:121:104091. doi: 10.1016/j.dci.2021.104091. Epub 2021 Apr 2.

Abstract

In mammals, Class II, major histocompatibility complex (MHC II) transactivator (CIITA) recognizes microbial pathogens and triggers immune responses. In Chinese tongue sole Cynoglossus semilaevis, Cs-CIITA was prevalently expressed in various tissues. Cs-CIITA, Cs-MHC IIA and Cs-MHC IIB were expressed significantly higher in skin in susceptible families infected with Vibrio harveyi, while higher expression of Cs-CIITA and Cs-MHC IIB was examined in liver in resistant families. In addition, the three genes were up-regulated in gill, skin, intestine, liver, spleen and kidney at 48 h or 72 h after V. harveyi infection. Furthermore, the three genes were co-expressed in the epithelial mucous cells of gill, skin, and intestine. Knockdown of Cs-CIITA regulates the expression of other inflammation-related genes, including CD40, IL-1β, IL-8, RelB, NFκB, and Myd88. These results suggest that CIITA functions in the inflammatory responses of C. semilaevis against V. harveyi, via MHC II transcriptional regulation.

Keywords: CIITA; Cynoglossus semilaevis; Immunity; MHC II; Vibrio harveyi.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence / genetics
  • Animals
  • Cloning, Molecular
  • Fish Diseases / immunology*
  • Fish Diseases / microbiology
  • Fish Proteins / genetics
  • Fish Proteins / metabolism*
  • Flatfishes / growth & development
  • Flatfishes / immunology*
  • Flatfishes / microbiology
  • Gene Expression Profiling
  • Gene Expression Regulation / immunology*
  • Gene Knockdown Techniques
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Phylogeny
  • Sequence Alignment
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Vibrio / immunology
  • Vibrio Infections / immunology*
  • Vibrio Infections / microbiology

Substances

  • Fish Proteins
  • MHC class II transactivator protein
  • Nuclear Proteins
  • Trans-Activators

Supplementary concepts

  • Vibrio harveyi