Generation of Human Neural Progenitors from Blood Samples by Interrupted Reprogramming

Eszter Szabó; Flóra Juhász; László Homolya; Zsuzsa Erdei; Ágota Apáti

doi:10.1007/7651_2021_386

Generation of Human Neural Progenitors from Blood Samples by Interrupted Reprogramming

Methods Mol Biol. 2022:2454:241-255. doi: 10.1007/7651_2021_386.

Authors

Eszter Szabó¹, Flóra Juhász¹, László Homolya¹, Zsuzsa Erdei¹, Ágota Apáti²

Affiliations

¹ Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary.
² Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary. [email protected].

PMID: 33826126
DOI: 10.1007/7651_2021_386

Abstract

Human neuronal cell cultures are essential tools for biological and preclinical studies of our nervous system. Since we have very limited access to primary human neural samples, derivation of proliferative neural progenitor cells (NPCs) from cells harvested by minimally invasive sampling is a key issue. Here we describe a "shortcut" method to establish proliferative NPC cultures directly from peripheral blood mononuclear cells (PBMCs) via interrupted reprogramming. In addition, we provide procedures to characterize the NPC stage.

Keywords: Calcium signaling; Interrupted reprogramming; Neural differentiation; Neural progenitors; Peripheral blood mononuclear cells; Sendai virus; Shortcut protocol.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / physiology
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Neural Stem Cells*
Neurons