First report of rice root-knot nematode, Meloidogyne graminicola, infecting Juncus microcephalus in Brazil

Cristiano Bellé; Paulo Sergio Dos Santos; Tiago Edu Kaspary

doi:10.21307/jofnem-2021-031

First report of rice root-knot nematode, Meloidogyne graminicola, infecting Juncus microcephalus in Brazil

J Nematol. 2021 Mar 20:53:e2021-31. doi: 10.21307/jofnem-2021-031. eCollection 2021.

Authors

Cristiano Bellé¹, Paulo Sergio Dos Santos², Tiago Edu Kaspary³

Affiliations

¹ Phytus Group, Estação Experimental de Itarra, Estrada da Estação, 3219, Interior, 97185-000, Itaara, RS, Brazil.
² Phytus Group, Estação Experimental de Planaltina, Rodovia DF, no. 145, Km 3, 73301-970, Planaltina, DF, Brazil.
³ Instituto Nacional de Investigación Agropecuaria - INIA La Estanzuela, Colonia, Uruguay.

Abstract

Juncus microcephalus plants showing symptoms of root-knot nematode infestation were observed in the municipality of Agudo, Rio Grande do Sul state, Brazil. Based on morphological observation, esterase phenotypes, and molecular analyses of rDNA-ITS and D2-D3 regions of 28S rDNA, the causal agent of the observed symptoms was identified as Meloidogyne graminicola. Pathogenicity of M. graminicola was confirmed by fulfilling modified Koch's postulates. To our knowledge, this is the first report of M. graminicola in J. microcephalus in Rio Grande do Sul State, Brazil.

Keywords: EST phenotype; Identification; Molecular analyses; Root-knot nematodes.