The Role of IgM Antibodies in T Cell Lymphoma Protection in a Novel Model Resembling Anaplastic Large Cell Lymphoma

J Immunol. 2021 May 15;206(10):2468-2477. doi: 10.4049/jimmunol.2001279. Epub 2021 Apr 21.

Abstract

MRL/lpr mice typically succumb to immune complex-mediated nephritis within the first year of life. However, MRL/lpr mice that only secrete IgM Abs because of activation-induced deaminase deficiency (AID-/-MRL/lpr mice) experienced a dramatic increase in survival. Further crossing of these mice to those incapable of making secretory IgM (μS mice) generated mice lacking any secreted Abs but with normal B cell receptors. Both strains revealed no kidney pathology, yet Ab-deficient mice still experienced high mortality. In this article, we report Ab-deficient MRL/lpr mice progressed to high-grade T cell lymphoma that can be reversed with injection of autoreactive IgM Abs or following adoptive transfer of IgM-secreting MRL/lpr B cells. Anti-nuclear Abs, particularly anti-dsDNA IgM Abs, exhibited tumor-killing activities against a murine T cell lymphoma cell line. Passive transfers of autoreactive IgM Abs into p53-deficient mice increased survival by delaying onset of T cell lymphoma. The lymphoma originated from a double-negative aberrant T cell population seen in MRL/lpr mice and most closely resembled human anaplastic large cell lymphoma. Combined, these results strongly implicate autoreactive IgM Abs in protection against T cell lymphoma.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adoptive Transfer / methods*
  • Animals
  • Antibodies, Antinuclear / administration & dosage*
  • Autoimmunity / genetics
  • B-Lymphocytes / immunology
  • Cytidine Deaminase / deficiency*
  • Cytidine Deaminase / genetics
  • Disease Models, Animal
  • Immunoglobulin M / administration & dosage*
  • Immunoglobulin M / deficiency*
  • Immunoglobulin M / genetics
  • Lymphoma, Large-Cell, Anaplastic / genetics
  • Lymphoma, Large-Cell, Anaplastic / immunology*
  • Lymphoma, Large-Cell, Anaplastic / therapy*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred MRL lpr
  • Mice, Knockout
  • T-Lymphocytes / immunology
  • Treatment Outcome
  • Tumor Suppressor Protein p53 / deficiency
  • Tumor Suppressor Protein p53 / genetics

Substances

  • Antibodies, Antinuclear
  • Immunoglobulin M
  • Trp53 protein, mouse
  • Tumor Suppressor Protein p53
  • anti-dsDNA autoantibody
  • anti-small nuclear ribonucleoproteins autoantibodies
  • secretory IgM
  • AICDA (activation-induced cytidine deaminase)
  • Cytidine Deaminase