[New technologies for renal pathology: Transcriptomics on paraffin-embedded fixed tissue]

Blaise Robin; Jessy Dagobert; Pierre Isnard; Marion Rabant; Jean-Paul Duong-Van-Huyen

doi:10.1016/j.nephro.2020.03.004

[New technologies for renal pathology: Transcriptomics on paraffin-embedded fixed tissue]

Nephrol Ther. 2021 Apr:17S:S54-S59. doi: 10.1016/j.nephro.2020.03.004.

[Article in French]

Authors

Blaise Robin¹, Jessy Dagobert², Pierre Isnard³, Marion Rabant³, Jean-Paul Duong-Van-Huyen⁴

Affiliations

¹ Paris Translational Research Center for Organ Transplantation, 56, rue Leblanc, 75015 Paris, France; Université de Paris, 56, rue Leblanc, 75015 Paris, France; Inserm U970, 56, rue Leblanc, 75015 Paris, France. Electronic address: [email protected].
² Paris Translational Research Center for Organ Transplantation, 56, rue Leblanc, 75015 Paris, France; Université de Paris, 56, rue Leblanc, 75015 Paris, France; Inserm U970, 56, rue Leblanc, 75015 Paris, France.
³ Service d'anatomie pathologique, hôpital Necker-Enfants-Malades, 149, rue de Sèvres, 75015 Paris, France.
⁴ Paris Translational Research Center for Organ Transplantation, 56, rue Leblanc, 75015 Paris, France; Université de Paris, 56, rue Leblanc, 75015 Paris, France; Inserm U970, 56, rue Leblanc, 75015 Paris, France; Service d'anatomie pathologique, hôpital Necker-Enfants-Malades, 149, rue de Sèvres, 75015 Paris, France.

PMID: 33910699
DOI: 10.1016/j.nephro.2020.03.004

Abstract

The development of new high-throughput technologies in genomics and then in transcriptomics has modified clinical approach in nephrology. At the interface between high-throughput technologies (microarray, new generation sequencing «NGS») and few mRNA analysis (reverse transcriptase quantitative PCR [RT-qPCR]), the nCounter® of NanoString® offers a new and complementary approach. Capable of analyzing formalin-fixed paraffin-embedded samples, this technology is a credible candidate for implanting transcriptomics in clinical routine.

Keywords: Kidney transplantation; Molecular pathology; Nephrology; Néphrologie; Pathologie moléculaire; Transcriptomic; Transcriptomique; Transplantation rénale.

Publication types

Review

MeSH terms

Humans
Kidney
Nephrology*
Paraffin Embedding
RNA, Messenger
Transcriptome*

Substances

RNA, Messenger