Human skin microbiota-friendly lysostaphin

Int J Biol Macromol. 2021 Jul 31:183:852-860. doi: 10.1016/j.ijbiomac.2021.04.154. Epub 2021 Apr 28.

Abstract

Growing antibiotic resistance of bacteria is a burning problem of human and veterinary medicine. Expansion and introduction of novel microbicidal therapeutics is highly desirable. However, antibiotic treatment disturbs the balance of physiological microbiota by changing its qualitative and/or quantitative composition, resulting in a number of adverse effects that include secondary infections. Although such dysbiosis may be reversed by the treatment with probiotics, a more attractive alternative is the use of antibiotics that target only pathogens, while sparing the commensals. Here, we describe lysostaphin LSp222, an enzyme produced naturally by Staphylococcus pseudintermedius 222. LSp222 is highly effective against S. aureus, including its multi-drug resistant strains. Importantly, the inhibitory concentration for S. epidermidis, the predominant commensal in healthy human skin, is at least two orders of magnitude higher compared to S. aureus. Such significant therapeutic window makes LSp222 a microbiota-friendly antibacterial agent with a potential application in the treatment of S. aureus-driven skin infections.

Keywords: Bacteriocin; Lysostaphin; Staphylococcus aureus.

MeSH terms

  • Drug Resistance, Bacterial / drug effects
  • Humans
  • Lysostaphin / pharmacology*
  • Methicillin-Resistant Staphylococcus aureus / drug effects
  • Microbial Sensitivity Tests
  • Microbiota / drug effects*
  • Skin / drug effects
  • Skin / microbiology*
  • Staphylococcus / enzymology*
  • Staphylococcus epidermidis / drug effects

Substances

  • Lysostaphin

Supplementary concepts

  • Staphylococcus pseudintermedius