A neutral water-soluble polysaccharide (RLP50-2) was extracted and purified from the fruits of Rosa laevigata. The absolute molecular weight was determined as 1.26 × 104 g/mol. Monosaccharide composition analysis showed that RLP50-2 mainly consisted of glucose, arabinose, and galactose. Structural analysis revealed that RLP50-2 consisted of →5)-α-L-Araf-(1→, →2,5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →6)-α-D-Glcp-(1→, →3,6)-β-D-Glcp-(1→, →4)-α-D-Galp-(1→, →6)-β-D-Galp-(1→, →2)-β-D-Xylp-(1→, terminal α-L-arabinose, and terminal β-D-mannose. Biological assays showed that RLP50-2 had immunomodulatory activities using cell and zebrafish models. Moreover, RLP50-2 showed significantly antitumor activities by inhibiting tumor cell proliferation and migration and blocking angiogenesis. These results suggested that RLP50-2 could be developed as a potential immunomodulatory agent or antitumor candidate drug in biomedicine field.
Keywords: 2′, 7′-Dichlorofluorescein diacetate (PubChem CID: 104913); 3-Amino, 4-aminomethyl-2′, 7′-difluorescein diacetate (PubChem CID: 2762646); Antitumor activity; Chloroform (PubChem CID: 6212); Dimethyl sulfoxide (PubChem CID: 679); Immunoregulatory activity; Methyl iodide (PubChem CID: 6328); Neutral polysaccharide; Rosa laevigata; Sodium borohydride (PubChem CID: 4311764); Sodium chloride (PubChem CID: 5234); Thiazolyl blue tetrazolium bromide (PubChem CID: 64965); Tricaine (PubChem CID: 261501); Trifluoroacetic acid (PubChem CID: 6422); Zebrafish xenograft model.
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