Human Acquired Aplastic Anemia Patients' Bone-Marrow-Derived Mesenchymal Stem Cells Are Not Influenced by Hematopoietic Compartment and Maintain Stemness and Immune Properties

Vandana Sharma; Sonali Rawat; Suchi Gupta; Sweta Tamta; Rinkey Sharma; Tulika Seth; Sujata Mohanty

doi:10.1155/2021/6678067

Human Acquired Aplastic Anemia Patients' Bone-Marrow-Derived Mesenchymal Stem Cells Are Not Influenced by Hematopoietic Compartment and Maintain Stemness and Immune Properties

Anemia. 2021 Apr 29:2021:6678067. doi: 10.1155/2021/6678067. eCollection 2021.

Authors

Vandana Sharma¹, Sonali Rawat², Suchi Gupta², Sweta Tamta², Rinkey Sharma², Tulika Seth¹, Sujata Mohanty²

Affiliations

¹ Department of Hematology, All India Institute of Medical Sciences, New Delhi 110029, India.
² Stem Cell Facility, DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi 110029, India.

Abstract

Methods: In the current study, we investigated the morphological differences, proliferation capacity, population doubling time (PDT), surface marker profiling, trilineage differentiation potential, and immunosuppressive ability of BM Mesenchymal Stem Cells (BM-MSCs) from untreated aAA patients and in the same number of age- and gender-matched controls.

Results: We observed similar morphology, proliferation capacity, phenotype, trilineage differentiation potential, and immunomodulatory properties of BM-MSCs in aAA patients and control subjects.

Conclusion: Our results confirm that the basic and immunosuppressive properties of BM-MSCs from aAA patients do not differ from normal BM-MSCs. Our data suggest that BM-MSCs from aAA patients might not be involved in disease pathogenesis. However, owing to a smaller number of samples, it is not conclusive, and future studies with more exhaustive investigation at transcriptome level are warranted.