Low-cost CRISPR diagnostics for resource-limited settings

Sneha Gulati; Souvik Maiti; Debojyoti Chakraborty

doi:10.1016/j.tig.2021.05.001

Low-cost CRISPR diagnostics for resource-limited settings

Trends Genet. 2021 Sep;37(9):776-779. doi: 10.1016/j.tig.2021.05.001. Epub 2021 May 17.

Authors

Sneha Gulati¹, Souvik Maiti², Debojyoti Chakraborty³

Affiliations

¹ CSIR-Institute of Genomics and Integrative Biology, Mathura Road, New Delhi, 110025, India.
² CSIR-Institute of Genomics and Integrative Biology, Mathura Road, New Delhi, 110025, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
³ CSIR-Institute of Genomics and Integrative Biology, Mathura Road, New Delhi, 110025, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India. Electronic address: [email protected].

PMID: 34016451
DOI: 10.1016/j.tig.2021.05.001

Abstract

Next-generation sequencing (NGS) has identified disease hallmarks and catalogued a vast reservoir of genetic information from humans and other species. Precise nucleotide-interrogation properties of clustered regularly interspaced short palindromic repeats (CRISPR) proteins have been harnessed to rapidly identify DNA-RNA signatures for diverse applications, bypassing the cost and turnaround times associated with diagnostic NGS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers, Tumor / genetics
CRISPR-Associated Proteins / genetics
Clustered Regularly Interspaced Short Palindromic Repeats*
DNA
Genetic Techniques* / economics
Humans
Molecular Diagnostic Techniques / methods*
Plants, Medicinal / genetics
RNA
Tuberculosis / diagnosis
Tuberculosis / microbiology

Substances

Biomarkers, Tumor
CRISPR-Associated Proteins
RNA
DNA