Isolation and Identification of Naturally Occurring Strigolactones

Kotomi Ueno; Takatoshi Wakabayashi; Yukihiro Sugimoto

doi:10.1007/978-1-0716-1429-7_2

Isolation and Identification of Naturally Occurring Strigolactones

Methods Mol Biol. 2021:2309:13-23. doi: 10.1007/978-1-0716-1429-7_2.

Authors

Kotomi Ueno¹, Takatoshi Wakabayashi², Yukihiro Sugimoto³

Affiliations

¹ Faculty of Agriculture, Tottori University, Koyama, Tottori, Japan.
² Graduate School of Agricultural Science, Kobe University, Nada, Kobe, Japan.
³ Graduate School of Agricultural Science, Kobe University, Nada, Kobe, Japan. [email protected].

PMID: 34028675
DOI: 10.1007/978-1-0716-1429-7_2

Abstract

The accurate structure determination of strigolactones (SLs) that are produced by plants leads to the precise understanding of the biosynthesis and functions of their molecules. SLs need to be isolated and purified from the plant roots or root exudates in a hydroponic solution using appropriate methods in order to determine the structures. In this chapter, we describe a small-scale extraction method for chromatographic analysis of known SLs and a large-scale purification method for isolation of unknown SLs, together with methods for the hydroponic culture of plants and collection of root exudates. Finally, we present spectroscopic data that are helpful in identifying SLs.

Keywords: Hydroponic culture; Preparative HPLC; Silica gel column chromatography; Solid-phase extraction; Spectroscopic analysis.

MeSH terms

Chromatography, High Pressure Liquid*
Heterocyclic Compounds, 3-Ring / isolation & purification*
Hydroponics
Lactones / isolation & purification*
Magnetic Resonance Spectroscopy
Molecular Structure
Plant Exudates / chemistry*
Plant Growth Regulators / isolation & purification*
Plant Roots / chemistry*
Solid Phase Extraction*
Spectrometry, Mass, Electrospray Ionization*
Spectrophotometry, Ultraviolet

Substances

GR24 strigolactone
Heterocyclic Compounds, 3-Ring
Lactones
Plant Exudates
Plant Growth Regulators