Effect of the Addition of Alginate and/or Tetracycline on Brushite Cement Properties

Molecules. 2021 May 28;26(11):3272. doi: 10.3390/molecules26113272.

Abstract

Calcium phosphate cements have the advantage that they can be prepared as a paste that sets in a few minutes and can be easily adapted to the shape of the bone defect, which facilitates its clinical application. In this research, six formulations of brushite (dicalcium phosphate dihydrated) cement were obtained and the effect of the addition of sodium alginate was analyzed, such as its capacity as a tetracycline release system. The samples that contain sodium alginate set in 4 or 5 min and showed a high percentage of injectability (93%). The cements exhibit compression resistance values between 1.6 and 2.6 MPa. The drug was released in a range between 12.6 and 13.2% after 7 days. The antimicrobial activity of all the cements containing antibiotics was proven. All samples reached values of cell viability above 70 percent. We also observed that the addition of the sodium alginate and tetracycline improved the cell viability.

Keywords: brushite; calcium phosphate cements; cell viability; mechanical properties; microbiological properties; physical-chemical properties.

MeSH terms

  • 3T3-L1 Cells
  • Alginates / chemistry*
  • Animals
  • Anti-Bacterial Agents / chemistry
  • Biocompatible Materials / chemistry
  • Bone Cements / chemistry*
  • Bone Regeneration
  • Calcium Phosphates / chemistry*
  • Cell Proliferation
  • Cell Survival
  • Compressive Strength
  • Hydrogen-Ion Concentration
  • Mice
  • Microscopy, Electron, Scanning
  • Microscopy, Electron, Transmission
  • Pressure
  • Stress, Mechanical
  • Tetracycline / chemistry
  • Tetracycline / pharmacology*
  • Time Factors
  • Tissue Engineering
  • X-Ray Diffraction

Substances

  • Alginates
  • Anti-Bacterial Agents
  • Biocompatible Materials
  • Bone Cements
  • Calcium Phosphates
  • Tetracycline
  • calcium phosphate, dibasic, dihydrate