Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system

Qian Luo; Cong Wei; Yan Long; Meng Zhang; Wei Shan; Honghu Li; Shuyang Cai; Yulin Xu; Pengxu Qian; He Huang

doi:10.1016/j.scr.2021.102350

Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system

Stem Cell Res. 2021 May:53:102350. doi: 10.1016/j.scr.2021.102350. Epub 2021 Apr 15.

Authors

Qian Luo¹, Cong Wei¹, Yan Long¹, Meng Zhang¹, Wei Shan¹, Honghu Li¹, Shuyang Cai¹, Yulin Xu¹, Pengxu Qian², He Huang³

Affiliations

¹ BoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR China.
² BoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR China; Center of Stem Cell and Regenerative Medicine, School of Medicine, Zhejiang University, Hangzhou 310012, PR China; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China. Electronic address: [email protected].
³ BoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR China; Center of Stem Cell and Regenerative Medicine, School of Medicine, Zhejiang University, Hangzhou 310012, PR China. Electronic address: [email protected].

PMID: 34087984
DOI: 10.1016/j.scr.2021.102350

Abstract

Human ELTD1 (Epidermal growth factor, latrophilin and seven-transmembrane domain-containing 1), an orphan G-protein-coupled receptor (GPCR) belonging to the adhesion GPCR family, has been reported as a novel regulator of angiogenesis and a potential anti-cancer therapeutic target. However, little is known about the function of ELTD1, especially its undiscovered ligands. In this experiment, an ELTD1 homozygous knockout human embryonic stem cell line, FAHZUe001-A, was generated by the iCRISPR/Cas9 system to achieve a deeper understanding of ELTD1. The FAHZUe001-A was confirmed with normal karyotype, typical undifferentiated morphology, pluripotency and trilineage differentiation potential in vitro.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics
Epidermal Growth Factor
Human Embryonic Stem Cells*
Humans
Neoplasms*
Neovascularization, Pathologic

Substances

Epidermal Growth Factor