Oncogenic BRAF, unrestrained by TGFβ-receptor signalling, drives right-sided colonic tumorigenesis

Nat Commun. 2021 Jun 8;12(1):3464. doi: 10.1038/s41467-021-23717-5.

Abstract

Right-sided (proximal) colorectal cancer (CRC) has a poor prognosis and a distinct mutational profile, characterized by oncogenic BRAF mutations and aberrations in mismatch repair and TGFβ signalling. Here, we describe a mouse model of right-sided colon cancer driven by oncogenic BRAF and loss of epithelial TGFβ-receptor signalling. The proximal colonic tumours that develop in this model exhibit a foetal-like progenitor phenotype (Ly6a/Sca1+) and, importantly, lack expression of Lgr5 and its associated intestinal stem cell signature. These features are recapitulated in human BRAF-mutant, right-sided CRCs and represent fundamental differences between left- and right-sided disease. Microbial-driven inflammation supports the initiation and progression of these tumours with foetal-like characteristics, consistent with their predilection for the microbe-rich right colon and their antibiotic sensitivity. While MAPK-pathway activating mutations drive this foetal-like signature via ERK-dependent activation of the transcriptional coactivator YAP, the same foetal-like transcriptional programs are also initiated by inflammation in a MAPK-independent manner. Importantly, in both contexts, epithelial TGFβ-receptor signalling is instrumental in suppressing the tumorigenic potential of these foetal-like progenitor cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / metabolism
  • Animals
  • Carcinogenesis / metabolism*
  • Carcinogenesis / pathology
  • Cell Differentiation
  • Cell Survival
  • Colon / pathology
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / metabolism*
  • Epithelial Cells / metabolism
  • Fetus / pathology
  • Inflammation / pathology
  • Kaplan-Meier Estimate
  • MAP Kinase Signaling System
  • Mice
  • Mice, Inbred C57BL
  • Mutation
  • Prognosis
  • Proto-Oncogene Proteins B-raf / genetics
  • Proto-Oncogene Proteins B-raf / metabolism*
  • Receptor, Transforming Growth Factor-beta Type I / metabolism
  • Receptors, Transforming Growth Factor beta / metabolism*
  • Signal Transduction*
  • Spheroids, Cellular / metabolism
  • Spheroids, Cellular / pathology
  • Transcription Factors / metabolism
  • Transforming Growth Factor beta / metabolism
  • Wnt Proteins / metabolism
  • Wnt Signaling Pathway
  • YAP-Signaling Proteins

Substances

  • Adaptor Proteins, Signal Transducing
  • Receptors, Transforming Growth Factor beta
  • Transcription Factors
  • Transforming Growth Factor beta
  • Wnt Proteins
  • YAP-Signaling Proteins
  • YAP1 protein, human
  • Proto-Oncogene Proteins B-raf
  • Receptor, Transforming Growth Factor-beta Type I
  • Tgfbr1 protein, mouse