Low miR-214-5p Expression Correlates With Aggressive Subtypes of Pediatric ALCL With Non-Common Histology

Piero Di Battista; Federica Lovisa; Enrico Gaffo; Ilaria Gallingani; Carlotta C Damanti; Anna Garbin; Lavinia Ferrone; Elisa Carraro; Marta Pillon; Luca Lo Nigro; Rossella Mura; Marco Pizzi; Vincenza Guzzardo; Angelo Paolo Dei Tos; Alessandra Biffi; Stefania Bortoluzzi; Lara Mussolin

doi:10.3389/fonc.2021.663221

Low miR-214-5p Expression Correlates With Aggressive Subtypes of Pediatric ALCL With Non-Common Histology

Front Oncol. 2021 May 25:11:663221. doi: 10.3389/fonc.2021.663221. eCollection 2021.

Authors

Piero Di Battista^{1

2}, Federica Lovisa^{1

2}, Enrico Gaffo³, Ilaria Gallingani^{1

2}, Carlotta C Damanti^{1

2}, Anna Garbin^{1

2}, Lavinia Ferrone^{1

2}, Elisa Carraro¹, Marta Pillon¹, Luca Lo Nigro⁴, Rossella Mura⁵, Marco Pizzi⁶, Vincenza Guzzardo⁶, Angelo Paolo Dei Tos⁶, Alessandra Biffi^{1

2}, Stefania Bortoluzzi^{3

7}, Lara Mussolin^{1

2}

Affiliations

¹ Division of Pediatric Hematology, Oncology and Stem Cell Transplant, Maternal and Child Health Department, University of Padova, Padova, Italy.
² Istituto di Ricerca Pediatrica Città della Speranza, Padova, Italy.
³ Department of Molecular Medicine, University of Padova, Padova, Italy.
⁴ Center of Pediatric Hematology Oncology, Azienda Policlinico G. Rodolico - San Marco, Catania, Italy.
⁵ Department of Paediatric Haematology-Oncology, Ospedale Pediatrico Microcitemico, Cagliari, Italy.
⁶ Surgical Pathology and Cytopathology Unit, Department of Medicine - DIMED, University of Padova, Padova, Italy.
⁷ CRIBI Interdepartmental Research Center for Innovative Biotechnologies (CRIBI), University of Padova, Padova, Italy.

Abstract

The unsatisfactory cure rate of relapsing ALK-positive Anaplastic Large-Cell Lymphoma (ALCL) of childhood calls for the identification of new prognostic markers. Here, the small RNA landscape of pediatric ALK-positive ALCL was defined by RNA sequencing. Overall, 121 miRNAs were significantly dysregulated in ALCL compared to non-neoplastic lymph nodes. The most up-regulated miRNA was miR-21-5p, whereas miR-19a-3p and miR-214-5p were reduced in ALCL. Characterization of miRNA expression in cases that relapsed after first line therapy disclosed a significant association between miR-214-5p down-regulation and aggressive non-common histology. Our results suggest that miR-214-5p level may help to refine the prognostic stratification of pediatric ALK-positive ALCL.

Keywords: ALCL; RNA-seq; biomarker; childhood; miRNA; prognosis.