Evaluation of Three Immunoassays for the Rapid Detection of SARS-CoV-2 antigens

Diagn Microbiol Infect Dis. 2021 Oct;101(2):115434. doi: 10.1016/j.diagmicrobio.2021.115434. Epub 2021 May 21.

Abstract

Three assays for SARS-CoV-2 antigen detection in nasopharyngeal swabs (Lumipulse® G SARS-CoV-2 Ag [LPG], STANDARDTM F COVID-19 Ag FIA [STF] and AFIAS COVID-19 Ag [AFC] were evaluated. Compared to RT-PCR, LPG, AFC and STF showed a variable sensitivity (87.9%, 37.5%, and 35.7%, respectively) and an overall high specificity (> 95%).

Keywords: COVID-19; SARS-CoV-2; antigen; chemiluminescent assay; lateral flow immunofluorescence assay.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Antigens, Viral / analysis
  • COVID-19 / diagnosis*
  • COVID-19 Serological Testing*
  • Humans
  • Immunoassay
  • Nasopharynx / virology
  • Point-of-Care Testing*
  • SARS-CoV-2 / immunology
  • SARS-CoV-2 / isolation & purification*
  • Sensitivity and Specificity

Substances

  • Antigens, Viral