Flow Cytometry Analysis of Mycobacteria and Mycobacteria-Infected Immune Cells

Sydney L Solomon; Bryan D Bryson

doi:10.1007/978-1-0716-1460-0_11

Flow Cytometry Analysis of Mycobacteria and Mycobacteria-Infected Immune Cells

Methods Mol Biol. 2021:2314:261-271. doi: 10.1007/978-1-0716-1460-0_11.

Authors

Sydney L Solomon^{1

2}, Bryan D Bryson^{3

4}

Affiliations

¹ Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
² Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
³ Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. [email protected].
⁴ Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA. [email protected].

PMID: 34235657
DOI: 10.1007/978-1-0716-1460-0_11

Abstract

Flow cytometry enables the measurement of tens of features on individual cells from complex mixtures. Flow cytometry enables high-throughput quantification of cell size, gene and protein expression. In the case of studies of host-pathogen interactions, this tool provides a facile way of identifying cells that have been successfully infected by a pathogen. Several recent technological advances have greatly improved throughput and the number of features that can be simultaneously monitored by this technique. Here, we describe common workflows to study Mycobacterium tuberculosis heterogeneity and host-M. tuberculosis interactions using flow cytometry and related technologies.

Keywords: Flow cytometry; Image cytometry; Immunology; Mass cytometry; Mycobacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Flow Cytometry / methods*
Host-Pathogen Interactions*
Humans
Macrophages / immunology*
Macrophages / microbiology*
Mycobacterium Infections / immunology
Mycobacterium Infections / microbiology*
Mycobacterium Infections / pathology
Mycobacterium tuberculosis / pathogenicity*