An electron donor-acceptor organic photoactive composite with Schottky heterojunction induced photoelectrochemical immunoassay

A signal enhancement photoelectrochemical (PEC) immunoassay system induced by the composite (PTCs@Au) of electron donor-acceptor with Schottky heterojunction was designed. Carcinoembryonic antigen (CEA) was selected as a model target. Initially, the capture anibody (Ab1) was linked to gold nanoparticles electrodeposited on glassy carbon electrode and sealed by bovine serum albumin. Meanwhile, the organic semiconductor (PTCs) with the structure of electron donor-acceptor was synthetized from perylene tetracarboxylic dianhydride (acceptor) and dopamine (donor) via amidation reaction. Then PTCs@Au composite with Schottky heterojunction was formed through gold nanoparticles in situ reduction and functionalization with PTCs. Next, the detection antibody was labeled by PTCs@Au composite (Ab2-PTCs@Au) as an immuno-probe. The PTCs@Au was introduced via sandwich immune reaction leading to enhancement PEC signal without additional electron donor nor acceptor for achieving quantitative detection of CEA under external light. The proposed immunoelectrode showed dynamic ranges of 0.5 fg mL^-1 to 10 pg mL^-1 and 10 pg mL^-1 to 1 μg mL^-1 with the detection limit of 0.17 fg mL^-1. In addition, this PEC strategy with acceptable selectivity and stability can be potentially applied to detect other targets by choosing appropriate target recognition unit.